In a notable advance within the realm of eco-friendly nanotechnology, a recent study has explored the utilization of Alstonia Scholaris, a widely recognized medicinal plant, for the production of copper nanoparticles (Cu-NPs). This research delves into the phytochemical properties of Alstonia Scholaris leaves extracted using various solvents and their subsequent application in the green synthesis of Cu-NPs.
The study commenced with a comprehensive phytochemical screening of the plant’s leaves using solvents like chloroform, ethyl acetate, n-hexane, methanol, and water. This screening revealed a rich presence of flavonoids and alkaloids, especially in the ethyl acetate and chloroform extracts. Interestingly, the methanol extract, devoid of proteins, anthraquinone, and reducing sugars, demonstrated a potent antibacterial activity against Escherichia coli and Staphylococcus aureus, the latter being more susceptible.
The research further capitalized on the methanol extract’s bioactivity to synthesize Cu-NPs, employing a green chemistry approach. Characterization of these nanoparticles through Fourier infrared spectroscopy (FT-IR), UV-visible spectroscopy, and scanning electron microscopy (SEM) confirmed their successful formation. The SEM images highlighted the irregular shapes of the nanoparticles, and energy-dispersive X-ray spectroscopy (EDX) analysis verified the predominance of copper with a relative abundance of 67.41 wt%, indicating the purity of the Cu-NPs colloids. 
